When working with BaseSpace Sequence Hub PrepTab, i5 sequences have to be entered into these program inside the ahead orientation in all situations and for all instrument kinds.
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Which means that these software package Focus on the idea that the i5 sequence is usually in the forward orientation and may quickly produce the reverse enhance of your sequences presented for the appropriate instrument sort.
Established D Index Adapters Refer to Index 2 (i5) Orientation for more information regarding how to enter i5 bases on the sample sheet in ahead or reverse complement orientation.
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For runs uploaded manually (i.e., an Illumina Software is just not being used to crank out the sample sheet), the index orientation is determined by read more which instrument that you are employing. Be sure to see the Index Orientation Table for info on the best way to enter the i5 sequence dependant upon the instrument and software package used.
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The MiniSeq Speedy made a recipe adjust that employs the grafted oligo for the i5 index priming, so it is actually read through in the forward orientation comparable to a MiSeq.
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When assessing index sets for color equilibrium, the sequence noticed with the instrument has to be assessed, regardless of the orientation employed for the sample sheet. For that i7 index, often asses coloration equilibrium using the "i7 bases for sample sheet" column orientation.
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